Product Category
Products > Biolab Use EnzymesEnterokinase ELISA Kit
Name:Enterokinase ELISA Kit
Description:The Kit is for the quantitative determination of bovine enterokinase in final product which is produced using enterokinase in manufacture process. The assay range is 40~1280pg/ml.
 

Description

        The kit uses anti Enterokinase antibody as the coating antibody, the enzyme labeled anti enterokinase antibody as the detection antibody, the highly sensitive TMB as the substrate, and the Enterokinase as the standard to quantitatively detect Enterokinase in the sample. This kit is characterized by good specificity, high sensitivity and stability,

        The lowest detectable level of Enterokinase is 0.1ng/ml.   

Principle

        This kit uses double antibody sandwich method to detect Enterokinase residuals. The Enterokinase antibody is coated on the microplate, and the Enterokinase in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled Enterokinase antibody is added for incubation, and the enzyme labeled antibody is combined with Enterokinase. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The Enterokinase concentration of the sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.

Kit components

Components

QTY

Package

Anti-Enterokinaseantibody coated microtiter plate

12 strips

96T

Enterokinase STD

5 vials

1ml/vial

Enterokinase reference

1 vial

1ml/vial

Sample dilution buffer

1 bottle

10ml/bottle

HRP-labeled antibody(100X)

1 vial

100μl/vial

HRP-labeled antibody dilution buffer

1 bottle

10ml/bottle

Substrate solution

1 bottle

10ml/bottle

Stop solution

1 bottle

10ml/bottle

Wash buffer concentrate(20X)

2 bottles

25ml/bottle

COA

1 copy

 

Manual

1 copy

 

 

Specificity

        Control abs(NSB)                      <0.300
        6.4ng/ml std abs                             >0.700
        Precision,%CV average <9.0%
        Detection limit (>control 3SD) <0.05ng/ml
 
Storage and stability

        Antibody-coated plate, HRP labeled antibody and Enterokinase standards are stored at -20℃, other components are stored at 2-8℃. It is stable for 6 months.

Catalog #

        TE-19-003