Description

        The kit uses anti RNase A antibody as the coating antibody, the enzyme labeled anti RNase A antibody as the detection antibody, the highly sensitive TMB as the substrate, and RNase A as the standard to quantitatively detect RNase A in the sample. This kit is characterized by good specificity, high sensitivity and stability,

        The lowest detectable level of RNase A is 0.2ng/ml.

Principle

        This kit uses double antibody sandwich method to detect RNase A residuals. The RNase A antibody is coated on the microplate, and the RNase A in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled RNase A antibody is added for incubation, and the enzyme labeled antibody is combined with RNase A. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The RNase A concentration of the sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.

Kit components

Specificity

        Antibody-coated plate, HRP labeled antibody and RNase A standards are stored at -20℃, other components are stored at 2-8℃. It is stable for 6 months.

Catalog #

        TE-19-009